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产品名称:pmirGLO质粒(ZK515)

货号 规格 价格 订购数量 是否现货
ZK515 1ug×1支 870 - + 有货
质粒分类
主要功能次级功能用途抗性
哺乳细胞质粒
哺乳报告质粒
双荧光素质粒

Amp


■ 基本信息

启动子:PGK,SV40

复制子:pUC ori,SV40 ori

终止子:SV40 poly(A) signal

质粒分类:哺乳细胞,信号通路报告载体

质粒大小:7350bp

原核抗性:Amp

克隆菌株:DH5α

培养条件:37℃,有氧 LB

表达宿主:哺乳细胞

诱导方式:无须诱导,瞬时表达

5'测序引物:Sv40-polyA-R:GAAATTTGTGATGCTATTGC

3'测序引物:根据序列设计引物


■ 质粒简介

The pmirGLO Dual-Luciferase miRNA Target Expression Vector(a-d) is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites 3' of the firefly luciferase gene (luc2). These target sites can be introduced by cloning putative miRNA binding sites alone, or the 3' untranslated region (UTR) of a gene of interest, to study the influence of these sites on transcript stability and activity. Firefly luciferase is the primary reporter gene; reduced firefly luciferase expression indicates the binding of endogenous or introduced miRNAs to the cloned miRNA target sequence. This vector is based on Promega dual-luciferase technology, with firefly luciferase (luc2) used as the primary reporter to monitor mRNA regulation and Renilla luciferase (hRluc-neo) acting as a control reporter for normalization and selection.


载体质粒图谱和多克隆位点信息