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产品名称:pRS316质粒(ZK958)

货号 规格 价格 订购数量 是否现货
ZK958 1ug×1支 1170 - + 有货

■ 基本信息

启动子:URA3,T7,T3

复制子:pUC ori,FI ori

质粒分类:酵母系列,酿酒酵母表达载体

质粒大小:4887bp

原核抗性:Amp

筛选标记:URA3

克隆菌株:DH5α

培养条件:37℃,有氧 LB

表达宿主:酵母细胞

诱导方式:半乳糖诱导

5'测序引物:T7:TAATACGACTCACTATAGGG

3'测序引物:M13R:CAGGAAACAGCTATGACC


质粒简介

This is one of a series of pBluescriptbased YCtype (centromeric) yeast shuttle vectors (ATCC 7714277145) differing in the yeast selectable marker gene. It encodes the beta galactosidase alpha peptide for blue white color detection of inserts and also has the T7 and T3 promoters for in vitro RNA synthesis and priming sites for sequencing. It is useful in plasmid shuffling experiments. It was constructed by inserting a 1.112 kb fragment containing the URA3 gene into the NdeI site of the pRSS56 vector and also inserting a cassette containing CEN6 and the ARS associated with histone 4 (ARSH4) into the AatII site of the same vector. All ends were blunted. The pRSS56 vector was constructed by ligating a PvuI fragment (bp 4982412) of pBluescript KS+ and the fragment from the unique NdeI and AatII sites between bla and f1 origin of pBS(+). The order of the major features in this plasmid is: URA3  f1 ori (NaeI)  T7 promoter  lacZ/MCS  T3 promoter pMB1 ori  bla  CEN6  ARSH4.


载体质粒图谱和多克隆位点信息