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产品名称:pGBKT7-53(ZK981)

货号 规格 价格 订购数量 是否现货
ZK981 1ug×1支 870 - + 有货

产品介绍:

Yeast two-hybrid systems provide a sensitive method for detecting relatively weak and transient protein interactions. Such interactions may not be biochemically detectable, but may be critical for proper functioning of complex biological systems. pGBKT7-53 and pGADT7-T encode fusions between the GAL4 DNA-BD and AD and murine p53 and SV40 large T-antigen, respectively. p53 and large T-antigen interact in a yeast two-hybrid assay.

基本信息:

启动子:ADH1,TRP1,T7

复制子:2μ ori,ori,FI ori

质粒分类:酵母系列,酵母双杂交载体

质粒大小:8.3kb

原核抗性:Kan

筛选标记:TRP1

克隆菌株:DH5α

培养条件:37℃,有氧 LB

表达宿主:酵母细胞

5'测序引物:T7:TAATACGACTCACTATAGGG

3'测序引物:根据序列设计引物


质粒图谱: