■ 基本信息
启动子: | ADH1 |
复制子: | 2μ |
质粒分类: | 酵母系列，酵母双杂交载体 |
质粒大小: | 7.9kb |
原核抗性: | Kan |
真核抗性: | TRP1 |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 酵母细胞 |
5'测序引物: | T7:TAATACGACTCACTATAGGG |
3'测序引物: | 根据序列设计引物 |
■ 质粒属性
质粒宿主: | 酵母菌 |
质粒用途: | 杂交 |
片段类型: |
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片段物种: |
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原核抗性: | Kan |
真核抗性: | TRP1
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荧光标记: |
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■ 质粒简介
pGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein (a.a. 66–230) and the GAL4 DNA-BD (a.a. 1–147). The lamin C cDNA insert (GenBank Accession #M13451) was derived from the plasmid referenced in Bartel et al. (1993a). Plasmid modification was performed atBD Biosciences Clontech. Yeast cotransformed with pGBKT7-Lam and pGADT7-RecT, provide a measure of the backgroundthat is due to false-positive two-hybrid interactions. pGBKT7-Lam has not been sequenced.
■ 质粒图谱
■ 质粒序列
质粒序列可参考（具体以测序为准）下载：
ZK982 pGBKT7-lam酵母双杂交质粒.txt
