庄盟生物

当前位置:首页 - 生物资源 - 生物资源

产品名称:Tuner(DE3) pLysS菌株

货号 规格 价格 订购数量 是否现货
ZK267 0.3ml/支 600 - + 有货

■ 产品介绍

Tuner strains are lacZY deletion mutants of BL21 and enable adjustable levels of protein expression throughout all cells in a culture. The lac permease (lacY) mutation allows uniform entry of IPTG into all cells in the population, which produces a concentration-dependent, homogeneous level of induction. By adjusting the concentration of IPTG, expression can be regulated from very low levels up to the robust, fully induced levels commonly associated with pET vectors. Lower-level expression may enhance the solubility and activity of difficult target proteins.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.

基因型:

F– ompT hsdSB (rB– mB–) gal dcm lacY1(DE3) pLysS (CamR)


■ 基本信息

抗性:

Chl

培养基:

LB

菌株类别:

大肠杆菌

培养条件:

37℃,有氧,LB

质粒转化:

42℃热激

保存方式:

20%甘油,-20℃

基本应用:

用于蛋白表达


■ 使用方法

建议超净台内划线涂板,得到单克隆后再进行其他实验操作,避免杂菌污染。


■ 注意事项

1. 本产品仅可用于实验室研究,不能用于动物,人体以及作为食品添加剂等用途。

2. 为了您的安全和健康,请穿实验服并戴一次性手套操作。

3. 使用甘油菌时可不完全融解,在甘油菌表面蘸取少量涂布固体琼脂平板即可,也可完全融解后使用,但随着冻融次数的增加,菌株的活力会逐渐下降。

用户姓名: *
用户手机: *
电子邮箱:
单位名称: *
留言内容: *
验证码: 看不清,换一张 *
 
订单提交
尊敬的用户,您好,请您再次确认以下订购商品,点击提交按钮,会提交到后台管理员,我们会在收到您的订购信息后,一小时内跟您电话确认,请保持电话畅通!(注册会员可享更多优惠!)
总价格:¥2000