■ 基本信息
复制子: | p15A |
质粒大小: | 3941bp |
原核抗性: | Amp,Kan |
克隆菌株: | DH5a |
培养条件: | 37度 |
5'测序引物: | 根据序列设计 |
3'测序引物: | 根据序列设计 |
备注: | 丰富的酶切位点 |
■ 质粒属性
质粒宿主: | 大肠杆菌 |
质粒用途: | 基因模板 |
片段类型: | ORF
|
片段物种: | 空载体
|
原核抗性: | Amp,Kan |
真核抗性: |
|
荧光标记: |
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■ 质粒简介
pACYC177 is an E. coli plasmid cloning vector containing the p15A origin of replication (1-4). This allows pACYC177 to coexist in cells with plasmids of the ColE1 compatibility group (e.g., pBR322, pUC19). It is a low copy number vector, at about 15 copies per cell (5), but can be amplified with chloramphenicol or spectinomycin.
Enzymes with unique restriction sites are shown in bold type and enzymes with two restriction sites are shown in regular type. Location of sites of all NEB restriction enzymes can be found on the NEB web site (choose Technical Reference > DNA Sequences and Maps). Restriction site coordinates refer to the position of the 5′-most base on the top strand in each recognition sequence.
■ 质粒图谱
■ 质粒序列
质粒序列可参考（具体以测序为准）下载：
ZK119pACYC177大肠克隆质粒.txt
