■ 基本信息
启动子: | T7 |
复制子: | pBR322 |
质粒分类: | 大肠杆菌载体；克隆质粒 |
质粒大小: | 2464bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培养条件: | 37度 |
5'测序引物: | Sp6:ATTTAGGTGACACTATAGAA |
3'测序引物: | T7:TAATACGACTCACTATAGGG |
备注: | 丰富的酶切位点 |
■ 质粒属性
质粒宿主: | 大肠杆菌 |
质粒用途: | 基因模板 |
片段类型: | cDNA
|
片段物种: | 空载体
|
原核抗性: | Amp |
真核抗性: |
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荧光标记: |
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■ 质粒简介
The pSP73 Vector offers a wide range of restriction sites, providing greater versatility in cloning and transcription of RNA in vitro. The pSP73 Vector contains the SP6 and T7 RNA polymerase promoters and a unique multiple cloning region, which includes restriction sites for BglII, EcoRV, ClaI, EcoRI, SacI, KpnI, SmaI, BamHI, XbaI, SalI, AccI, PstI, SphI, HindIII, PvuII and XhoI. The pSP72 and pSP73 Vectors are essentially identical except for the orientation of the multiple cloning region.
质粒只保证关键序列正确，不保证表达效果。
■ 质粒图谱
■ 质粒序列
质粒序列可参考（具体以测序为准）下载：
ZK123pSP73体外转录质粒.txt
