■ 基本信息
启动子: | SV40，T7，T3 |
复制子: | pBR322 |
质粒分类: | 哺乳细胞，蛋白过表达载体 |
质粒大小: | 7.9kb |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物: | T7:TAATACGACTCACTATAGGG |
3'测序引物: | BGH:TAGAAGGCACAGTCGAGG |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF
|
片段物种: | 空载体 |
原核抗性: | Amp |
真核抗性: | G418
|
荧光标记: |
|
■ 质粒简介
SuperCos 1 is a novel, 7.9-kb cosmid vector that contains bacteriophage promoter sequences flanking a unique cloning site (see Figure 1). This structure allows rapid synthesis of "walking" probes specific for the extreme ends of insert DNA. The SuperCos 1 vector is also engineered to contain genes for the amplification and expression of cosmid clones in eukaryotic cells. In addition, most genomic inserts can be excised as a single large restriction fragment using the Not I restriction site that flanks the SuperCos 1 polylinker.
质粒只保证关键序列正确，不保证表达效果。
■ 质粒图谱
