■ 基本信息
别名: | Plasmid #63890 |
启动子: | Lac/lac promoter |
复制子: | pUC |
质粒大小: | 4991bp |
质粒标签: | C-6×HIS，C-HA |
原核抗性: | Amp |
克隆菌株: | TG1 |
培养条件: | 37度 |
■ 质粒属性
质粒宿主: | 大肠杆菌,噬菌体 |
质粒用途: | 蛋白表达,抗体表达,噬菌体展示 |
片段类型: | ORF
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片段物种: | 空载体
|
原核抗性: | Amp |
真核抗性: |
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荧光标记: |
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■ 质粒简介
pComb3XSS是一个噬菌体展示质粒。pComb3X is the newest of the pComb vectors. Improvements over pComb3 include increased stability and introduction of an asymmetric SfiI cassette for directional cloning of full Fab, scFv, peptide and other protein for phage display. 6xHis and HA tags allow for purification and detection. An amber stop codon was introduced to turn-off expression of the pIII fusion protein by switching to a non-supressor strain of E. coli allowing production of soluble protein without subcloning. Alternatively, the gene for phage protein pIII can be removed by SpeI/NheI digest. pComb3XSS is recommended for preparation of vector for library cloning. The “SS” refers to the double stuffer, a 1200bp stuffer in the Fab light chain cloning region bounded by SacI and XbaI restriction sites and a 300bp stuffer in Fab heavy chain cloning region bound by XhoI and SpeI restriction sites. Also, the 1600bp double stuffer (both stuffers plus the leader sequence between the Fab light chain and heavy chain cloning regions) can be removed by SfiI digest so that non-Fab genes of interest can be cloned. Also available on Addgene: pComb3XTT and pComb3XLambda are only needed at templates for the construction of chimeric Fab libraries as described in Phage Display: A Laboratory Manual. pComb3XTT can also be used as an Fab expression control. 3rd generation plasmid for phage display on modified geneIII, contains stuffer fragment.
质粒只保证关键序列正确，不保证表达效果。
■ 质粒图谱
■ 质粒序列
质粒序列可参考（具体以测序为准）下载：
ZK129pComb3XSS噬菌体展示质粒.txt
