■ 基本信息
别名: | pGEX6P1;pGEX6P-1 |
启动子: | Tac |
复制子: | pBR322 |
质粒分类: | 大肠杆菌载体;PGEX系列表达质粒 |
质粒大小: | 4984bp |
质粒标签: | N-GST,N-PP/3C |
原核抗性: | Amp |
克隆菌株: | DH5a |
培养条件: | 37℃,有氧,LB |
表达宿主: | 大肠杆菌 |
诱导方式: | IPTG或乳糖及其类似物 |
5'测序引物: | pGEX5:GGGCTGGCAAGCCACGTTTGGTG |
3'测序引物: | pGEX3:CCGGGAGCTGCATGTGTCAGAGG |
备注: | 可用GST亲和柱纯化重组蛋白 |
■ 质粒属性
质粒宿主: | 大肠杆菌 |
质粒用途: | 蛋白表达 |
片段类型: | ORF
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片段物种: | 空载体
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原核抗性: | Amp |
真核抗性: |
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荧光标记: |
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■ 质粒简介
There are a range of Gluthatione Sepharose™ prepacked column and bulk media products available to purify GST Fusion proteins. For manual purification of sample volumes up to 600 μl use GST SpinTrap™ microspin columns or GST MultiTrap™ 4B 96-well plates. For sample volumes between 600 μl and 10 ml use GST GraviTrap™ gravity flow column. Where sample volumes are above 10 ml, use LabMate™ reservoir together with GST GraviTrap. All formats described can be used for preparation of samples in parallel. In addition GST HiTrap™ 1 and 5 ml columns and GST HiPrep™ FF 16/10 column are available for purification in a chromatography system such as the ÄKTA™ design system. Alternatively, Gluthatione Sepharose bulk media are available from 10 ml up to 500 ml. A GST Bulk Kit is also available combining 10 ml Gluthatione Sepharose 4B bulk medium and 5 empty gravity flow columns with required buffers. For simplified buffer preparation use the GST Buffer Kit. Ordering information for all associated products is listed below.
质粒只保证关键序列正确,不保证表达效果。
■ 质粒图谱
■ 质粒序列
质粒序列请下载:ZK137pGEX-6P-1大肠表达质粒.txt