庄盟生物

当前位置:首页 - 生物资源 - 生物资源

产品名称:pGEX-6P-1质粒

货号 规格 价格 订购数量 是否现货
ZK137 1μg(20μl,50ng/μl) 870 - + 有货

基本信息

别名:

pGEX6P1;pGEX6P-1

启动子:

Tac

复制子:

pBR322

质粒分类:

大肠杆菌载体;PGEX系列表达质粒

质粒大小:

4984bp

质粒标签:

N-GST,N-PP/3C

原核抗性:

Amp

克隆菌株:

DH5a

培养条件:

37℃,有氧,LB

表达宿主:

大肠杆菌

诱导方式:

IPTG或乳糖及其类似物

5'测序引物:

pGEX5:GGGCTGGCAAGCCACGTTTGGTG

3'测序引物:

pGEX3:CCGGGAGCTGCATGTGTCAGAGG

备注:

可用GST亲和柱纯化重组蛋白


质粒属性

质粒宿主:

大肠杆菌

质粒用途:

蛋白表达

片段类型:

ORF

片段物种:

空载体

原核抗性:

Amp

真核抗性:

荧光标记:



质粒简介

There are a range of Gluthatione Sepharose™ prepacked column and bulk media products available to purify GST Fusion proteins. For manual purification of sample volumes up to 600 μl use GST SpinTrap™ microspin columns or GST MultiTrap™ 4B 96-well plates. For sample volumes between 600 μl and 10 ml use GST GraviTrap™ gravity flow column. Where sample volumes are above 10 ml, use LabMate™ reservoir together with GST GraviTrap. All formats described can be used for preparation of samples in parallel. In addition GST HiTrap™ 1 and 5 ml columns and GST HiPrep™ FF 16/10 column are available for purification in a chromatography system such as the ÄKTA™ design system. Alternatively, Gluthatione Sepharose bulk media are available from 10 ml up to 500 ml. A GST Bulk Kit is also available combining 10 ml Gluthatione Sepharose 4B bulk medium and 5 empty gravity flow columns with required buffers. For simplified buffer preparation use the GST Buffer Kit. Ordering information for all associated products is listed below.

质粒只保证关键序列正确,不保证表达效果。

质粒图谱


质粒序列

质粒序列请下载:ZK137pGEX-6P-1大肠表达质粒.txt

用户姓名: *
用户手机: *
电子邮箱:
单位名称: *
留言内容: *
验证码: 看不清,换一张 *
 
订单提交
尊敬的用户,您好,请您再次确认以下订购商品,点击提交按钮,会提交到后台管理员,我们会在收到您的订购信息后,一小时内跟您电话确认,请保持电话畅通!(注册会员可享更多优惠!)
总价格:¥2000