■ 基本信息
别名: | pET3a |
启动子: | T7 |
复制子: | pBR322 |
终止子: | T7 terminator |
质粒大小: | 4640bp |
质粒标签: | N-T7 |
原核抗性: | Amp |
克隆菌株: | DH5a |
培养条件: | 37℃，有氧，LB |
表达宿主: | 大肠杆菌BL21(DE3) |
诱导方式: | 无需诱导 |
5'测序引物: | T7:TAATACGACTCACTATAGGG |
3'测序引物: | T7-ter:TGCTAGTTATTGCTCAGCGG |
备注: | 组成型表达 |
■ 质粒属性
质粒宿主: | 大肠杆菌 |
质粒用途: | 蛋白表达 |
片段类型: | ORF
|
片段物种: | 空载体
|
原核抗性: | Amp |
真核抗性: |
|
荧光标记: |
|
■ 质粒简介
pET-3a载体是一个大肠杆菌表达载体，T7启动子驱动T7标签和目的基因融合表达。该载体是组成型表达，并且不携带6×HIS标签。The pET-3a-d vectors carry an N-terminal T7•Tag sequence and BamH I cloning site. These vectors are the precursors to many pET family vectors; the pET-23a-d(+) series corresponds to pET-3a-d but incorporates several additional features. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. 质粒只保证关键序列正确，不保证表达效果。
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK141pET-3a大肠表达质粒.txt
