■ 基本信息
别名: | pET21a |
启动子: | T7 |
复制子: | pBR322 |
终止子: | T7 terminator |
质粒分类: | 大肠杆菌载体；PET系列表达质粒 |
质粒大小: | 5443bp |
质粒标签: | N-T7, C-His |
原核抗性: | Amp |
克隆菌株: | DH5a |
培养条件: | 37℃，有氧，LB |
表达宿主: | 大肠杆菌BL21(DE3) |
诱导方式: | IPTG或乳糖及其类似物 |
5'测序引物: | T7:TAATACGACTCACTATAGGG |
3'测序引物: | T7-ter:TGCTAGTTATTGCTCAGCGG |
■ 质粒属性
质粒宿主: | 大肠杆菌 |
质粒用途: | 蛋白表达 |
片段类型: | ORF
|
片段物种: | 空载体
|
原核抗性: | Amp |
真核抗性: |
|
荧光标记: |
|
■ 质粒简介
pET-21a质粒是一个大肠杆菌表达载体，T7启动子驱动T7标签、6×HIS标签和目的蛋白融合表达。The pET-21a-d(+) vectors carry an N-terminal T7•Tag® sequence plus an optional C-terminal His•Tag® sequence. These vectors differ from pET-24a-d(+) only by their selectable marker (ampicillin vs. kanamycin resistance). Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer.质粒只保证关键序列正确，不保证表达效果。
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK151pET-21a大肠表达质粒.txt
