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产品名称:pET-43.1b质粒

货号 规格 价格 订购数量 是否现货
ZK183 1μg(20μl,50ng/μl) 870 - + 有货

基本信息

别名:

pET43.1b

启动子:

T7

复制子:

pBR322

终止子:

T7 terminator

质粒分类:

大肠杆菌载体;PET系列表达质粒

质粒大小:

7274bp

质粒标签:

C-HSV, N-6×His, C-6×His, N-Thrombin, N-Nus, N-EK

原核抗性:

Amp

克隆菌株:

DH5a

培养条件:

37℃,有氧,LB

表达宿主:

BL21(DE3)

诱导方式:

IPTG或乳糖及其类似物

5'测序引物:

T7:TAATACGACTCACTATAGGG

3'测序引物:

T7-ter:TGCTAGTTATTGCTCAGCGG


质粒属性

质粒宿主:

大肠杆菌

质粒用途:

蛋白表达

片段类型:

ORF

片段物种:

空载体

原核抗性:

Amp

真核抗性:

荧光标记:



质粒简介

The pET-43.1 series of vectors are designed for cloning and high-level expression of peptide sequences fused with the 491 aa Nus•Tag™ protein. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/ expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the ColiDOWN primer. Vector encoded sequence can be completely removed when cloning into the PshA I or Sma I sites (as shown below) by cleaving the Nus•Tag fusion protein with enterokinase or thrombin, respectively.

质粒只保证关键序列正确,不保证表达效果。

质粒图谱


质粒序列

质粒序列请下载:ZK183pET-43.1b大肠表达质粒.txt

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总价格:¥2000