■ 基本信息
启动子: | T7 |
复制子: | M13 |
质粒分类: | 大肠杆菌载体；蛋白表达质粒 |
质粒大小: | 7477bp |
质粒标签: | Intein |
原核抗性: | Amp |
克隆菌株: | DH5a |
培养条件: | 37℃，有氧，LB |
表达宿主: | 大肠杆菌 |
5'测序引物: | T7:TAATACGACTCACTATAGGG |
3'测序引物: | 根据序列设计 |
备注: | 表达质粒 |
■ 质粒属性
质粒宿主: | 大肠杆菌 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 空载体 |
原核抗性: | Amp |
真核抗性: |
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荧光标记: |
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■ 质粒简介
The pTYB1 is an E. coli vector created for fusion protein expression and purification. A target gene can be inserted into the the ORF by use of the MCS upstream of the Sce VMA intein. The NdeI site in the MCS has the initiator sequence ATG that enables translation of the ORF. The chitin-binding domain(CBD) downstream of the intein results in a target protein-intein with C-terminal chitin beads that facilitate protein purification. Release of the protein is induced by the thiol cleavage activity of the intein.
质粒只保证关键序列正确，不保证表达效果。
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK335pTYB1大肠表达质粒.txt
