■ 基本信息
别名: | pCDNA3.1(-)-Myc-HisA |
启动子: | CMV |
复制子: | pUC |
终止子: | BGH poly（A）signal |
质粒分类: | 哺乳细胞载体;蛋白过表达质粒 |
质粒大小: | 5522bp |
质粒标签: | His Tag (6x), C-Myc Epitope Tag |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培养条件: | 37℃，有氧，LB |
表达宿主: | 哺乳细胞 |
5'测序引物: | T7 Fwd:5d[TAATACGACTCACTATAGGG]3 |
3'测序引物:
| 根据序列设计
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备注: |
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■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF
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片段物种: | 空载体
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原核抗性: | Amp |
真核抗性: | G418
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荧光标记: |
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■ 质粒简介
pcDNA3.1/myc-His(-) A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. The vectors are supplied in three reading frames to facilitate in frame cloning, with a C-terminal peptide containing a polyhistidine metal-binding tag and the myc (c-myc) epitope. The human cytomegalovirus immediate-early (CMV) promoter provides highlevel expression in a wide range of mammalian cells. In addition, the vector will replicate episomally in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7). High-level stable and nonreplicative transient expression can be carried out in most mammalian cells. The control plasmid, pcDNA3.1/myc-His(-)/lacZ, is the pcDNA3.1/myc-His(-) A vector with a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide. It is included for use as a positive control for transfection, expression, and detection in the cell line of choice.
质粒只保证关键序列正确，不保证表达效果。
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK357pCDNA3.1-Myc-HisA(-)哺乳表达质粒.txt
