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产品名称:pCDNA3.1-Myc-HisB(-)质粒

货号 规格 价格 订购数量 是否现货
ZK358 1μg(20μl,50ng/μl) 870 - + 有货

基本信息

别名:

pCDNA3.1(-)-Myc-HisB

启动子:

CMV

复制子:

pUC

终止子:

BGH poly(A)signal

质粒分类:

哺乳细胞载体;蛋白过表达质粒

质粒大小:

5520bp

质粒标签:

6X His, myc

原核抗性:

Amp

真核抗性:

G418

克隆菌株:

DH5a

培养条件:

37度

表达宿主:

哺乳细胞


质粒属性

质粒宿主:

哺乳细胞

质粒用途:

蛋白表达

片段类型:

ORF

片段物种:

空载体

原核抗性:

Amp

真核抗性:

G418

荧光标记:



质粒简介

pcDNA3.1/myc-His(-) A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. The vectors are supplied in three reading frames to facilitate in frame cloning, with a C-terminal peptide containing a polyhistidine metal-binding tag and the myc (c-myc) epitope. The human cytomegalovirus immediate-early (CMV) promoter provides highlevel expression in a wide range of mammalian cells. In addition, the vector will replicate episomally in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7). High-level stable and nonreplicative transient expression can be carried out in most mammalian cells. The control plasmid, pcDNA3.1/myc-His(-)/lacZ, is the pcDNA3.1/myc-His(-) A vector with a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide. It is included for use as a positive control for transfection, expression, and detection in the cell line of choice.  

质粒只保证关键序列正确,不保证表达效果。

质粒图谱


质粒序列

质粒序列请下载:ZK358pCDNA3.1-Myc-HisB(-)哺乳表达质粒.txt

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总价格:¥2000