■ 基本信息
启动子: | CMV |
复制子: | pUC |
终止子: | SV40 poly(A) signal,hGH poly(A) signal |
质粒分类: | 哺乳细胞，蛋白过表达载体 |
质粒大小: | 6310bp |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物:
| CMV-F：CGCAAATGGGCGGTAGGCGTG |
3'测序引物: | hGH-PA-R:CCAGCTTGGTTCCCAATAGA |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF
|
片段物种: | 空载体
|
原核抗性: | Amp |
真核抗性: | G418 |
荧光标记: |
|
■ 质粒简介
P3xFlag-CMV-14 expression vector is a 6.3 KB carrier derived from the pCMV5 vector, which is used for transient expression in mammalian cells or the stable expression of C terminal with 3xFlag tag fusion protein. Three adjacent Flag epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) were encoded by the downstream fragment of the vector. This makes it possible to detect protein expression using M2 Flag antibody.
质粒只保证关键序列正确，不保证表达效果。
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK379p3×FLAG-CMV-14哺乳表达质粒.txt
