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产品名称:p3×FLAG-CMV-13质粒

货号 规格 价格 订购数量 是否现货
ZK380 1μg(20μl,50ng/μl) 870 - + 有货

基本信息

启动子:

CMV

复制子:

pUC

终止子:

SV40 poly(A) signal,hGH poly(A) signal

质粒分类:

哺乳细胞,蛋白过表达载体

质粒大小:

6362bp

原核抗性:

Amp

真核抗性:

G418

克隆菌株:

DH5a

培养条件:

37度

表达宿主:

哺乳细胞

诱导方式:

无须诱导,瞬时表达

5'测序引物:
CMV-F:CGCAAATGGGCGGTAGGCGTG

3'测序引物:

hGH-PA-R:CCAGCTTGGTTCCCAATAGA


质粒属性

质粒宿主:

哺乳细胞

质粒用途:

蛋白表达

片段类型:

ORF

片段物种:

空载体

原核抗性:

Amp

真核抗性:

G418

荧光标记:



质粒简介

The p3XFLAG-CMV-13 Expression Vector is a 6.3kb derivative of pCMV5 used to establish expression of transient or unstable C-terminal 3XFLAG fusion proteins in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) downstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG-fusion constructs. The preprotrypsin leader sequence precedes the FLAG sequence. The aminoglycoside phosphotransferase II gene (Neo) confers resistance to aminoglycosides such as G 418, allowing for selection of stable transfectants. p3XFLAG-CMV-13 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using an SV40 T antigen-expressing host, such as COS cells.

质粒只保证关键序列正确,不保证表达效果。

质粒图谱


质粒序列

质粒序列请下载:ZK380p3×FLAG-CMV-13哺乳表达质粒.txt

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总价格:¥2000