■ 基本信息
启动子: | CMV |
复制子: | pUC |
终止子: | SV40 poly(A) signal,hGH poly(A) signal |
质粒分类: | 哺乳细胞，蛋白过表达载体 |
质粒大小: | 6362bp |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物:
| CMV-F：CGCAAATGGGCGGTAGGCGTG
|
3'测序引物: | hGH-PA-R:CCAGCTTGGTTCCCAATAGA |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF
|
片段物种: | 空载体
|
原核抗性: | Amp |
真核抗性: | G418
|
荧光标记: |
|
■ 质粒简介
The p3XFLAG-CMV-13 Expression Vector is a 6.3kb derivative of pCMV5 used to establish expression of transient or unstable C-terminal 3XFLAG fusion proteins in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) downstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG-fusion constructs. The preprotrypsin leader sequence precedes the FLAG sequence. The aminoglycoside phosphotransferase II gene (Neo) confers resistance to aminoglycosides such as G 418, allowing for selection of stable transfectants. p3XFLAG-CMV-13 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using an SV40 T antigen-expressing host, such as COS cells.
质粒只保证关键序列正确，不保证表达效果。
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK380p3×FLAG-CMV-13哺乳表达质粒.txt
