■ 基本信息
别名: | pCAGGS-HA-N |
启动子: | CAG |
复制子: | pUC |
质粒分类: | 哺乳系列质粒；哺乳表达质粒；pCAG系列质粒 |
质粒大小: | 4807bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培养条件: | 37℃，5%CO2 |
表达宿主: | 293T等哺乳细胞 |
诱导方式: | 无需诱导 |
5'测序引物: | pcaggs-F（GCTAACCATGTTCATGCCTTCT） |
3'测序引物: | pcaggs-R（TATGTCCTTCCGAGTGAGAG） |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 空载体 |
原核抗性: | Amp |
真核抗性: |
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荧光标记: |
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■ 质粒简介
pCAGGS-HA质粒是在pCAGGS载体的基础上添加了HA标签，利于在WB实验的时候用抗体检测。This plasmid is useful for highly efficient expression of genes under the control of the AG promoter and the human CMV-IE enhancer in various mammalian cells.The AG promoter sequence consists of the chicken β-actin promoter, the first exon and part of the first intron (that seems to have a strong enhancer-like activity) linked to a rabbit β-globin fragment, consisting of a 3' part of the second intron (inclusive a branch point which is required for normal splicing reactions) and a 5' part of the third exon.When cloning a fragment downstream from the lac promoter it may be advisable to use lacIq strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
质粒只保证关键序列正确，不保证表达效果。
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK401pCAGGS-HA哺乳表达质粒.txt
