■ 基本信息
启动子: | EF1a |
复制子: | pUC |
终止子: | BGH poly（A）signal |
质粒分类: | 哺乳细胞载体;蛋白过表达质粒 |
质粒大小: | 6174bp |
质粒标签: | 6X His, V5 |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培养条件: | 37℃，有氧，LB |
表达宿主: | 哺乳细胞 |
5'测序引物: | T7 Fwd : 5 |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 空载体 |
原核抗性: | Amp |
真核抗性: | G418 |
荧光标记: |
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■ 质粒简介
All pEF or pUB vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag. 质粒只保证关键序列正确，不保证表达效果。
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK405pEF1-V5-HisA哺乳表达质粒.txt
