■ 基本信息
别名: | pGL3.0-Enhancer |
复制子: | pUC |
终止子: | SV40 poly(A) signal |
质粒分类: | 哺乳细胞，信号通路报告载体 |
质粒大小: | 5064bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物: | RVP3:CTAGCAAAATAGGCTGTCCC |
3'测序引物:
| 根据序列设计引物
|
备注: | 哺乳细胞启动子报告对照质粒 |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 信号报告 |
片段类型: | Promoter
|
片段物种: |
|
原核抗性: | Amp |
真核抗性: |
|
荧光标记: | Fluc
|
■ 质粒简介
The pGL3 Luciferase Reporter Vectors(a–c) provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors. The backbone of the pGL3 Luciferase Reporter Vectors is designed for increased expression, and contains a modified coding region for firefly (Photinus pyralis) luciferase that has been optimized for monitoring transcriptional activity in transfected eukaryotic cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition, these Luciferase Reporter Vectors contain numerous features aiding in the structural characterization of the putative regulatory sequences under investigation.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK470pGL3-Enhancer哺乳报告质粒.txt
