■ 基本信息
启动子: | SV40 |
复制子: | pUC ori |
终止子: | SV40 poly(A) signal |
质粒分类: | 哺乳细胞，信号通路报告载体 |
质粒大小: | 4641bp |
原核抗性: | Amp |
克隆菌株: | 大肠杆菌DH5α |
培养条件: | 37℃，有氧 LB |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物: | RVP3:CTAGCAAAATAGGCTGTCCC |
3'测序引物: | 根据序列设计引物 |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 信号报告 |
片段类型: | Promoter |
片段物种: | 空载体 |
原核抗性: | Amp |
真核抗性: |
|
荧光标记: | Fluc
|
■ 质粒简介
The pGL4 Luciferase Reporter Vectors are the next generation of reporter gene vectors optimized for expression in mammalian cells. Numerous configurations of pGL4 Vectors are available, including those with the synthetic luc2 (Photinus pyralis) and hRluc (Renilla reniformis) genes, which have been codon optimized for more efficient expression in mammalian cells. Furthermore, both the reporter genes and the vector backbone, including the bla (β-lactamase or Ampr) gene, have been engineered to reduce the number of consensus transcription factor binding sites, reducing background and the risk of anomalous transcription.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK474pGL4.13[luc2-SV40]哺乳报告质粒.txt
