■ 基本信息
复制子: | pUC |
终止子: | SV40 poly(A) signal |
质粒分类: | 哺乳细胞，信号通路报告载体 |
质粒大小: | 4283bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物: | 根据序列设计引物 |
3'测序引物: | 根据序列设计引物 |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 信号报告 |
片段类型: | Promoter |
片段物种: | 空载体 |
原核抗性: | Amp |
真核抗性: |
|
荧光标记: | Fluc
|
■ 质粒简介
The pGL4.23[luc2/minP] Vector(a–e)encodes the luciferase reporter gene luc2 (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. The pGL4.23[luc2/minP] Vector contains a multiple cloning region for insertion of a response element of interest upstream of a minimal promoter and a luc2 gene. luc2 is a synthetically-derived luciferase sequence with humanized codon optimization. The vector backbone contains an ampicillin resistance gene to allow for selection in E. coli.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK477pGL4.23[luc2-minP]哺乳报告质粒.txt
