■ 基本信息
启动子: | SV40 |
复制子: | pUC |
终止子: | SV40 poly(A) signal |
质粒分类: | 哺乳细胞，信号通路报告载体 |
质粒大小: | 6084bp |
原核抗性: | Amp |
真核抗性: | Hyg |
克隆菌株: | DH5a |
培养条件: | 37℃ |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物:
| RVP3:CTAGCAAAATAGGCTGTCCC
|
3'测序引物: | 根据序列设计引物 |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 信号报告 |
片段类型: | Promoter
|
片段物种: | 空载体
|
原核抗性: | Amp |
真核抗性: | Hyg
|
荧光标记: | Fluc
|
■ 质粒简介
The pGL4.29[luc2P/CRE/Hygro] Vector(a–f) contains a cAMP response element (CRE) that drives the transcription of the luciferase reporter gene luc2P (Photinus pyralis). luc2P is a synthetically-derived luciferase sequence with humanized codon optimization that is designed for high expression and reduced anomalous transcription. The luc2P gene contains hPEST, a protein destabilization sequence. The protein encoded by luc2P responds more quickly than the protein encoded by the luc2 gene upon induction. The vector backbone contains an ampicillin resistance gene to allow selection in E. coli and a mammalian selectable marker for hygromycin resistance.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK479pGL4.29[luc2P-CRE-Hygro]哺乳报告质粒.txt
