■ 基本信息
启动子: | SV40 |
复制子: | pUC |
终止子: | SV40 poly(A) signal |
质粒分类: | 哺乳细胞，信号通路报告载体 |
质粒大小: | 3705bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物: | T7:TAATACGACTCACTATAGGG |
3'测序引物: | 根据序列设计引物 |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 信号报告 |
片段类型: |
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片段物种: |
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原核抗性: | Amp |
真核抗性: |
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荧光标记: | Fluc
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■ 质粒简介
The pRL-SV40 Vector may be used in combination with any experimental reporter vector to cotransfect mammalian cells. However, it is important to realize that trans effects between promoters on cotransfected plasmids can potentially affect reporter gene expression. This is primarily of concern when either the control or experimental reporter vector, or both, contain very strong promoter/enhancer elements. The occurrence and magnitude of such effects will depend on several factors: i) the combination and activities of the genetic regulatory elements present on the cotransfected vectors, ii) the relative ratio of experimental vector to control vector introduced into the cells, and iii) the cell type transfected.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK488pRL-SV40哺乳报告质粒.txt
