■ 基本信息
启动子: | CMV |
复制子: | pUC |
终止子: | SV40 poly(A) signal |
质粒分类: | 哺乳细胞，信号通路报告载体 |
质粒大小: | 6470bp |
原核抗性: | Amp |
真核抗性: | Puro |
克隆菌株: | DH5a |
培养条件: | 37℃ |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物: | CMV-F：CGCAAATGGGCGGTAGGCGTG |
3'测序引物: | M13F：TGTAAAACGACGGCCAGT |
备注: | 信号报告空载体 |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 信号报告 |
片段类型: | miRNA,UTR
|
片段物种: | 空载体
|
原核抗性: | Amp |
真核抗性: | Puro
|
荧光标记: | Fluc
|
■ 质粒简介
The pMIR-REPORT miRNA Expression Reporter Vector System consists of an experimental firefly luciferase reporter vector and an associated β-gal reporter control plasmid. By inserting predicted miRNA target sequences in the multiple cloning site, pMIR-REPORT Luciferase miRNA Expression Reporter Vector can be used to conduct accurate, quantitative evaluations of miRNA function. It can also be used to evaluate siRNA target sites and to analyze the influence of 3' UTR sequences on gene expression. pMIR-REPORT Luciferase contains a firefly luciferase reporter gene under the control of a CMV promoter/termination system. The 3' UTR of the luciferase gene contains a multiple cloning site for insertion of predicted miRNA binding targets or other nucleotide sequences. By cloning a predicted miRNA target sequence into pMIR-REPORT, the luciferase reporter is subjected to regulation that mimics the miRNA target.
本质粒的参考文献：Evaluation of the circulating MicroRNA‐495 and Stat3 as prognostic and predictive biomarkers for lower extremity deep venous thrombosis.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK492pMIR-Report Luciferase哺乳报告质粒.txt
