■ 基本信息
别名: | pmir-GLO |
启动子: | PGK |
复制子: | pUC |
终止子: | SV40 poly(A) signal |
质粒分类: | 哺乳细胞，信号通路报告载体 |
质粒大小: | 7350bp |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物: | PmirGLO-F：GACGAGGTGCCTAAAGGACT |
3'测序引物:
| 根据序列设计引物
|
备注: | 信号报告空载体 |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 信号报告 |
片段类型: | miRNA,UTR
|
片段物种: | 空载体
|
原核抗性: | Amp |
真核抗性: | G418 |
荧光标记: | Fluc,Rluc
|
■ 质粒简介
The pmirGLO Dual-Luciferase miRNA Target Expression Vector(a–d) is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites 3´ of the firefly luciferase gene (luc2). These target sites can be introduced by cloning putative miRNA binding sites alone, or the 3´ untranslated region (UTR) of a gene of interest, to study the influence of these sites on transcript stability and activity. Firefly luciferase is the primary reporter gene; reduced firefly luciferase expression indicates the binding of endogenous or introduced miRNAs to the cloned miRNA target sequence. This vector is based on Promega dual-luciferase technology, with firefly luciferase (luc2) used as the primary reporter to monitor mRNA regulation and Renilla luciferase (hRluc-neo) acting as a control reporter for normalization and selection.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK515pmirGLO哺乳报告质粒.txt
