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产品名称:pBIFC-bFosVC155

货号 规格 价格 订购数量 是否现货
ZK523 1μg(20μl,50ng/μl) 870 - + 有货

基本信息

别名:

Plasmid: #22013

启动子:

CMV

复制子:

pUC

终止子:

SV40 poly(A) signal

质粒分类:

哺乳细胞,双分子荧光互补载体

质粒大小:

4352bp

原核抗性:

Amp

克隆菌株:

DH5a

培养条件:

37度

表达宿主:

哺乳细胞

诱导方式:

无须诱导,瞬时表达

5'测序引物:
CMV-F:CGCAAATGGGCGGTAGGCGTG

3'测序引物:

根据序列设计引物


质粒属性

质粒宿主:

哺乳细胞

质粒用途:

信号报告

片段类型:


片段物种:


原核抗性:

Amp

真核抗性:

荧光标记:



质粒简介

Bimolecular fluorescence complementation (BiFC) analysis enables direct visualization of protein interactions in living cells. The BiFC assay is based on the discoveries that two non-fluorescent fragments of a fluorescent protein can form a fluorescent complex and that the association of the fragments can be facilitated when they are fused to two proteins that interact with each other. BiFC must be confirmed by parallel analysis of proteins in which the interaction interface has been mutated. The BiFC assay has been used for the visualization of interactions between many types of proteins in different subcellular locations and in different cell types and organisms. It is technically straightforward and can be performed using a regular fluorescence microscope and standard molecular biology and cell culture reagen


质粒图谱


质粒序列

质粒序列请下载:ZK523pBIFC-bFosVC155双分荧光质粒.txt

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总价格:¥2000