■ 基本信息
启动子: | CMV |
复制子: | pUC |
终止子: | SV40 poly(A) signal |
质粒分类: | 哺乳系列质粒；哺乳荧光质粒；哺乳红色质粒 |
质粒大小: | 4757bp |
质粒标签: | N-DsRed2 |
原核抗性: | Kan |
真核抗性: | G418 |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 293T等哺乳细胞 |
诱导方式: | 无需诱导，瞬时表达 |
5'测序引物: | CMV-F（CGCAAATGGGCGGTAGGCGTG） |
3'测序引物:
| SV40-polyA-R（GAAATTTGTGATGCTATTGC）
|
备注: | 哺乳细胞内质网标记质粒 |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 定位 |
片段类型: |
|
片段物种: |
|
原核抗性: | Kan |
真核抗性: | G418
|
荧光标记: |
|
■ 质粒简介
pDsRed2-ER是一种哺乳动物表达载体，用于标记活细胞内质网。DsRed2基因是按人的密码子优化过的野生型DsRed变体，可以更快的成熟和较低的非特异性聚集。
The vector encodes a fusion consisting of Discosoma sp. red fluorescent protein; the endoplasmic reticulum (ER) targeting sequence of calreticulin , fused to the 5' end of DsRed2; and the ER retention sequence, KDEL, fused to the 3' end of DsRed2. DsRed2 is a human codon-optimized variant of wild-type DsRed that has been engineered for faster maturation and lower non-specific aggregation.To drive expression of DsRed2, this vector contains the immediate early promoter of cytomegalovirus (PCMV IE). SV40 polyadenylation signals downstream of the DsRed2 gene direct proper processing of the 3'-end of the DsRed2 mRNA transcript. The vector also contains an SV40 origin for replication in any mammalian cell line that expresses the SV40 Tantigen, a pUC origin of replication for propagation in E. coli, and an f1 origin for single-stranded DNA production. A neomycin resistance cassette—consisting of the SV40 early promoter (PSV40e), the neomycin/kanamycin resistance gene of Tn5 (Neor/Kanr), and polyadenylation signals from the herpes simplex virus thymidine kinase (HSV TK poly A) gene— allows stably transfected eukaryotic cells to be selected using G418 . A bacterial promoter (P) upstream of this cassette drives expression of the gene encoding kanamycin resistance in E. coli.pDsRed2-ER can be introduced into mammalian cells using any standard transfection method. If required, stable transformants can be selected using G418 .
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK525pDsRed2-ER内质网定位质粒.txt
