■ 基本信息
启动子: | CMV |
复制子: | pUC |
终止子: | SV40 poly(A) signal |
质粒分类: | 哺乳细胞，荧光蛋白报告载体 |
质粒大小: | 4918bp |
原核抗性: | Kan |
真核抗性: | G418 |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物:
| CMV-F：CGCAAATGGGCGGTAGGCGTG
|
3'测序引物: | 根据序列设计引物 |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 定位 |
片段类型: |
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片段物种: |
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原核抗性: | Kan |
真核抗性: | G418
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荧光标记: |
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■ 质粒简介
pEYFP-Golgi encodes a fusion protein consisting of enhanced yellow fluorescent protein (EYFP) and a sequence encoding the N-terminal 81 amino acids of human beta 1,4-galactosyltransferase (GT). This region of human beta 1,4-GT contains the membrane-anchoring signal peptide that targets the fusion protein to the trans-medial region of the Golgi apparatus. The EYFP gene contains the four amino acid substitutions previously published as GFP-10C. The fluorescence excitation maximum of EYFP is 513 nm; the emission spectrum has a peak at 527 nm (in the yellow-green region). When excited at 514 nm, the Em of EYFP is 84,500 cm-1M-1 and the fluorescence quantum yield is 0.61, resulting in a bright fluorescent signal. The fluorescence observed is roughly equivalent to that of EGFP.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK533pEYFP-Golgi高尔基体定位质粒.txt
