■ 基本信息
启动子: | CMV |
复制子: | pUC |
终止子: | SV40 poly(A) signal |
质粒分类: | 哺乳细胞，哺乳细胞双杂交载体 |
原核抗性: | Amp |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物: | CMV-F：CGCAAATGGGCGGTAGGCGTG |
3'测序引物: | 根据序列设计引物 |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 杂交 |
片段类型: |
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片段物种: |
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原核抗性: | Amp |
真核抗性: |
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荧光标记: |
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■ 质粒简介
The protein product of the Id cDNA sequence in the pBIND-Id Vector is known to interact with the protein product of the MyoD cDNA sequence in the pACT-MyoD Vector.
■ 质粒图谱
■ 质粒序列
GGCTCGTTTAGTGACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCA
CAGTTAAATTGCTAACGCAGTCAGTGCTTCTGACACGACAGTCTCGAACTTAA
GCTGCAGTGACTCTCTTAAGGTAGCCTTGCAGAAGTTGGTCGTGAGGCACTGG
GCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAAAAACTGG
GCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTT
ACTGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTA
CAGCTCTTAAGGCTAGAGTACTTAATACGACTCACTATAGGCTAGCCAGCTTG
AAGCAAGCCTCCTGAAAGATGAAGCTACTGTCTTCTATCGAACAAGCATGCGA
TATTTGCCGACTTAAAAAGCTCAAGTGCTCCAAAGAAAAACCGAAGTGCGCCA
AGTGTCTGAAGAACAACTGGGAGTGTCGCTACTCTCCCAAAACCAAAAGGTCT
CCGCTGACTAGGGCACATCTGACAGAAGTGGAATCAAGGCTAGAAAGACTGGA
ACAGCTATTTCTACTGATTTTTCCTCGAGAAGACCTTGACATGATTTTGAAAA
TGGATTCTTTACAGGATATAAAAGCATTGTTAACAGGATTATTTGTACAAGAT
AATGTGAATAAAGATGCCGTCACAGATAGATTGGCTTCAGTGGAGACTGATAT
GCCTCTAACATTGAGACAGCATAGAATAAGTGCGACATCATCATCGGAAGAGA
GTAGTAACAAAGGTCAAAGACAGTTGACTGTATCGCCGGAATTCCCGGGGATC
CGTCGACATAAATTCCCACTTGGTCTGTCAGAGCAAAGCGTGGCCATCTCGCG
CTGCGCTGGGACGCGCCTGCCCGCCTTGCTGGACGAGCAGCAGTGAACGTCCT
GCTCTACGACATGAACGGCTGCTACTCACGCCTCAAGGAGCTGGTG
