■ 基本信息
启动子: | CMV |
复制子: | pUC |
终止子: | SV40 poly(A) signal |
质粒分类: | 哺乳细胞，哺乳细胞双杂交载体 |
质粒大小: | 7400bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物: | CMV-F：CGCAAATGGGCGGTAGGCGTG |
3'测序引物: | 根据序列设计引物 |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 杂交 |
片段类型: |
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片段物种: |
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原核抗性: | Amp |
真核抗性: |
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荧光标记: |
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■ 质粒简介
The CheckMate™ Mammalian Two-Hybrid System provides positive control vectors that encode and express two proteins known to interact in vivo (13). MyoD is expressed in skeletal muscle and is a myogenic regulatory protein (14,15). The Id protein acts as a negative regulator of myogenic differentiation (16). MyoD and Id are members of the helix-loop-helix family of nuclear proteins. The pBIND-Id Control Vector contains a segment of murine Id cDNA (amino acids 29–148; 13,16) cloned in-frame with the GAL4 DNA-binding domain of the pBIND Vector . The pACT-MyoD Control Vector contains a segment of murine MyoD cDNA (amino acids 1–318; 13,15) cloned in-frame with the VP16 activation domain of the pACT Vector .
■ 质粒图谱
