■ 基本信息
别名: | 乳酸传感 |
启动子: | CMV |
复制子: | pUC |
终止子: | SV40 poly(A) signal,bGH poly(A) signal |
质粒分类: | 哺乳细胞，乳酸生物传感器 |
质粒大小: | 7648bp |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物: | CMV-F：CGCAAATGGGCGGTAGGCGTG |
3'测序引物: | BGH:TAGAAGGCACAGTCGAGG |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF
|
片段物种: |
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原核抗性: | Amp |
真核抗性: | G418
|
荧光标记: |
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■ 质粒简介
Lactate is shuttled between and inside cells, playing metabolic and signaling roles in healthy tissues. Lactate is also a harbinger of altered metabolism and participates in the pathogenesis of inflammation, hypoxia/ischemia, neurodegeneration and cancer. Many tumor cells show high rates of lactate production in the presence of oxygen, a phenomenon known as the Warburg effect, which has diagnostic and possibly therapeutic implications. In this article we introduce Laconic, a genetically-encoded Forster Resonance Energy Transfer (FRET)-based lactate sensor designed on the bacterial transcription factor LldR. Laconic quantified lactate from 1 µM to 10 mM and was not affected by glucose, pyruvate, acetate,
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK555pcDNA3.1-Laconic乳酸传感质粒.txt
