■ 基本信息
别名: | PB513B-1；pB-CMV-MCS-ef1α-greenpuro |
启动子: | CMV |
复制子: | pUC |
终止子: | SV40 poly(A) signal |
质粒分类: | 哺乳系列质粒；哺乳编辑质粒；哺乳转座质粒 |
质粒大小: | 7258bp |
原核抗性: | Amp |
真核抗性: | Puro |
克隆菌株: | Stbl3 |
培养条件: | 37℃，5%CO2 |
表达宿主: | 293T等哺乳细胞 |
5'测序引物: | CMV-F（CGCAAATGGGCGGTAGGCGTG） |
3'测序引物: | Sv40-polyA-R（GAAATTTGTGATGCTATTGC） |
备注: | 哺乳细胞转座表达载体 |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达,转座 |
片段类型: | ORF
|
片段物种: | 空载体
|
原核抗性: | Amp |
真核抗性: | Puro
|
荧光标记: | 绿色
|
■ 质粒简介
PiggyBac Dual promoter (PB513B-1)是用于哺乳细胞转座实验的质粒，位于CMV启动子下游的多克隆位点（MCS），使目的基因或microRNA更方便克隆。下游的EF-1α core promoter启动GFP的表达。
The PiggyBac (PB) transposon is a mobile genetic element that efficiently transposes between vectors and chromosomes via a "cut and paste" mechanism. During transposition, the PB transposase recognizes transposon-specific inverted terminal repeat sequences (ITRs) located on both ends of the transposon vector and efficiently moves the contents from the original sites and efficiently integrates them into TTAA chromosomal sites. The powerful activity of the piggyBac transposon system enables genes of interest between the two ITRs in the PB vector to be easily mobilized into target genomes.
The unique features of piggyBac transposons are that there is NO Cargo Limit and it is also Reversible. Genomes containing an inserted piggyBac vector can be transiently re-transfected with the PB transposase expression vector. The PB transposase will remove the transposons from the genome, footprint-free.The Super PiggyBac transposase transient expression vector and PB513B-1 were co-transfected into HeLa cells and puromycin selection applied for 10 days (10ug/ml). Cells efficiently transposed were Puro resistant and GFP positive.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK564PiggyBac Dual promoter (PB513B-1)哺乳转座质粒.txt
