■ 基本信息
别名: | PB200PA-1 |
启动子: | Polr2A |
复制子: | pUC |
终止子: | SV40 poly(A) signal |
质粒分类: | 哺乳细胞转座酶表达质粒 |
质粒大小: | 8531bp |
原核抗性: | Amp |
克隆菌株: | Stbl3 |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物: | M13R:CAGGAAACAGCTATGACC |
3'测序引物: | 根据序列设计引物 |
备注: | 转座酶哺乳细胞表达质粒 |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达,转座 |
片段类型: | ORF
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片段物种: |
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原核抗性: | Amp |
真核抗性: |
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荧光标记: |
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■ 质粒简介
Super PiggyBac Transposase (PB200PA-1)是一个哺乳细胞转座质粒。The PiggyBac (PB) transposon is a mobile genetic element that efficiently transposes between vectors andchromosomes via a "cut and paste" mechanism. During transposition, the PB transposase recognizes transposonspecific inverted terminal repeat sequences (ITRs) located on both ends of the transposon vector and efficiently moves the contents from the original sites and efficiently integrates them into TTAA chromosomal sites. The powerful activity of the piggyBac transposon system enables genes of interest between the two ITRs in the PB vector to be easily mobilized into target genomes.The Super PiggyBac transposase expression vector features a 5’ HS4 insulator to support robust transcription from the rPolr2A promoter. The PiggyBac transposase coding sequence has been optimized for high expression, stability and activity in mammalian cells.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK565Super PiggyBac Transposase (PB200PA-1) 哺乳转座质粒.txt
