■ 基本信息
启动子: | CMV |
复制子: | pUC |
终止子: | SV40 poly(A) signal,BGH poly(A) signal |
质粒大小: | 5159bp |
原核抗性: | Amp |
真核抗性: | Zeo |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物: | T7:TAATACGACTCACTATAGGG |
3'测序引物: | BGH:TAGAAGGCACAGTCGAGG |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达,分泌表达 |
片段类型: | ORF |
片段物种: | 空载体 |
原核抗性: | Amp |
真核抗性: | Zeo
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荧光标记: |
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■ 质粒简介
pSecTag2 A, B, and C vectors are fusion vectors requiring that you clone your gene of interest in frame with the initiation ATG of the N-terminal Igκ-chain leader sequence and/or the C-terminal myc epitope/polyhistidine tag. Three versions of this vector are provided to facilitate cloning. For proper expression,first determine which restriction sites are appropriate for ligation and then which vector will preserve the reading frame at BOTH the 5′ and the 3′ ends. It may be nece ssary to PCR your gene product to create a fragment with the appropriate restriction sites to clone in frame at both ends. Carefully inspect your gene and the multiple cloning site of each vector before cloning your gene of interest.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK690pSecTag2A哺乳分泌质粒.txt
