■ 基本信息

■ 质粒属性

■ 质粒简介

pTRE2pur is a response plasmid that expresses a gene of interest (Gene X) in Clontech's Tet-On® and Tet-Off® Gene Expression Systems and Tet-On and Tet-Off Cell Lines (1). The Tet Expression Systems and Cell Lines give researchers ready access to the tetracycline-regulated expression systems described by Gossen & Bujard (2; Tet-Off) and Gossen et al. (3; Tet-On).pTRE2pur contains an MCS immediately downstream of the Tet-responsive PhCMV*-1 promoter. cDNAs or genes inserted into the MCS will be responsive to the tTA and rtTA regulatory proteins in the Tet-Off and Tet-On systems, respectively. PhCMV*-1 contains the Tet response element (TRE), which consists of seven copies of the 19-bp tet operator sequence (tetO). TheTRE element is just upstream of the minimal CMV promoter (Pmin CMV), which lacks the enhancer that is part of the complete CMV promoter. Consequently, PhCMV*-1 is silent in the absence of binding of TetR or rTetR to the tetO sequences. Note that the cloned insert must have an initiation codon. In some cases the addition of a Kozak consensus ribosome binding site (4) may improve expression levels; however, many cDNAs have been efficiently expressed in Tet systems without the addition of a Kozak sequence. The puromycin resistance gene is used to directly select for stable transformants.

■ 质粒图谱

■ 质粒序列

质粒序列请下载：ZK692pTRE2pur哺乳调控质粒.txt