■ 基本信息
启动子: | CMV |
复制子: | pUC |
终止子: | SV40 poly(A) signal，beta-globin polyA signal |
质粒分类: | 哺乳细胞，四环素调控系统载体 |
原核抗性: | Amp |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
诱导方式: | 四环素诱导 |
5'测序引物: | CMV-F：CGCAAATGGGCGGTAGGCGTG |
3'测序引物: | 根据序列设计引物 |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: |
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片段物种: |
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原核抗性: | Amp |
真核抗性: |
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荧光标记: |
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■ 质粒简介
pTet-Off expresses the tet-responsive transcriptional activator (tTA) from the strong immediate early promoter of cytomegalovirus (PCMV). tTA is a fusion of amino acids 1–207 of the tet repressor (TetR) and the negatively charged C-terminal activation domain (130 amino acids) of the VP16 protein of herpes simplex virus. pTet-Off was originally described as pUHD15-1neo by Resnitzky et al. (1994) and was created by insertion of a neomycin resistance gene into pUHD15-1 (Gossen & Bujard,1992). pTet-Off can be distinguished from pTet-On by digestion with Hind III.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK695pTet-Off哺乳调控质粒.txt
