■ 基本信息
启动子: | EF1α |
复制子: | pUC |
终止子: | SV40 poly(A) signal |
质粒分类: | 哺乳细胞，抗体表达载体 |
质粒大小: | 4194bp |
原核抗性: | Zeo |
真核抗性: | Zeo |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物: | 根据序列设计引物 |
3'测序引物: | 根据序列设计引物 |
备注: | 哺乳细胞抗体表达载体 |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF
|
片段物种: | 空载体
|
原核抗性: | Zeo |
真核抗性: | Zeo
|
荧光标记: |
|
■ 质粒简介
pFUSE-Fc is a family of plasmid developed to facilitate the construction of Fc-fusion proteins by fusing the effector region of a protein to the Fc region of an immunoglobul in G (IgG).
pFUSE-Fc plasmids yield high levels of Fc-fusion proteins. The level of expression is usually in the μg/mL range. They can be transfected in a variety of mammalian cells, including myeloma cell lines, CHO cells, monkey COS cells and human embryonic kidney (HEK)293 cells, cells that are commonly used in protein purification systems.
pFUSE-Fc2 (IL2ss) plasmids allow the secretion of Fc-Fusion proteins.They contain the IL2 signal sequence (IL2ss) for the generation of Fc- Fusion proteins derived from proteins that are not naturally secreted. As Fc- Fusion proteins are secreted, they can be easily detected in the supernatant of pFUSE-Fc-transfected cells by SDS-PAGE. Furthermore, functional domains can be identified by immunoblotting and ligand blotting.Fc-Fusion proteins can be easily purified by single-step protein A or protein G affinity chromatography.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK703pFUSE-hIgG1e1-Fc2哺乳抗体质粒.txt
