■ 基本信息
启动子: | Lac |
复制子: | pUC |
质粒分类: | 哺乳细胞，蛋白过表达载体 |
质粒大小: | 4392bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物: | M13R:CAGGAAACAGCTATGACC |
3'测序引物: | 根据序列设计引物 |
■ 质粒属性
质粒宿主: | 哺乳细胞 |
质粒用途: | 辅助包装 |
片段类型: |
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片段物种: |
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原核抗性: | Amp |
真核抗性: |
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荧光标记: |
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■ 质粒简介
The use of pAdVAntage vectors were transfected into mammalian cells can promote the initiation of translation to enhance the instantaneous protein expression in many cell types.
Transfection of mammalian cells with expression vector often leads to the expression of the target protein. The double stranded RNA (dsRNA), which is produced in the transfection process, is thought to activate the dsRNA- activation inhibitor (DAI), which is one of the many kinds of enzymes involved in the host cell antiviral defense system. DAI phosphorylation of translation initiation factor eIF-2, termination of translation and generation of protein. However, with the pAdVAntage vector were transfected into DAI in the process of translation inhibition by RNA polymerase III generated adeno-associated virus type I (RNA VAI RNA) offset. RNA DAI combined with VAI to prevent its activation, so as to complete the translation and protein expression.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK706pAdVAntage哺乳辅助质粒.txt
