■ 基本信息
启动子: | CMV |
复制子: | pUC |
终止子: | hGH poly(A) signal |
质粒分类: | 病毒系列,腺病毒相关克隆载体 |
质粒大小: | 5904bp |
原核抗性: | Amp |
克隆菌株: | Stbl3 |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导,瞬时表达 |
5'测序引物: | CMV-F:CGCAAATGGGCGGTAGGCGTG |
3'测序引物: | hGH-PA-R: CCAGCTTGGTTCCCAATAGA |
■ 质粒属性
质粒宿主: | 哺乳细胞,腺相关病毒 |
质粒用途: | 蛋白表达 |
片段类型: |
|
片段物种: |
|
原核抗性: | Amp |
真核抗性: |
|
荧光标记: | 绿色
|
■ 质粒简介
The pAAV-ZsGreen1 Vector is an adeno-associated virus (AAV) vector that contains the ZsGreen1 gene. This vector can be used as a positive control to monitor AAV particle production using AAVpro Helper Free Systems. Expression of ZsGreen, a variant of the green fluorescent protein from Zoanthus sp., can be used to assess transfection and transduction efficiency during AAV particle preparation.
■ 质粒图谱
■ 质粒序列
质粒序列请下载:ZK732pAAV-IRES-ZsGreen1腺相关质粒.txt