■ 基本信息
别名: | pAAV-RC2;pAAV-2 |
复制子: | pUC |
质粒分类: | 腺相关病毒包装质粒 |
质粒大小: | 7327bp |
原核抗性: | Amp |
克隆菌株: | Stbl3 |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
5'测序引物: | M13F:TGTAAAACGACGGCCAGT |
3'测序引物: | M13R:CAGGAAACAGCTATGACC |
■ 质粒属性
质粒宿主: | 哺乳细胞,腺相关病毒 |
质粒用途: | 辅助包装 |
片段类型: |
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片段物种: |
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原核抗性: | Amp |
真核抗性: |
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荧光标记: |
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■ 质粒简介
pAAV-RC2是一个腺相关病毒包装质粒，携带了Cap和Rep基因，可与pAAV-MCS和pHelper质粒一起包装成腺相关病毒。
pAAV-MCS克隆质粒：http://www.zomanbio.com/shengwu_info.php?nid=2314&id=141
pAAV-RC2包装质粒：http://www.zomanbio.com/shengwu_info.php?nid=2319&id=141
pHelper包装质粒：http://www.zomanbio.com/shengwu_info.php?nid=2320&id=141
This 7.3 kb vector harbors the cap and rep genes that make the capsid and DNA replication proteins required to make infectious virions. This vector is cotransfected into the packaging cell line for production of recombinant, replication-deficient AAV virions.The pAAV-RC plasmid contains the AAV-2 rep and cap genes, encoding replication proteins and viral capsid structural proteins, respectively. Establishing the proper expression levels of rep and cap gene products is an important step in achieving high-titer virus production. pAAV-RC directs Rep and Cap expression from two different promoters, achieving optimal expression levels for each set of gene products.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK735pAAV-RC腺相关病毒包装质粒.txt
