■ 基本信息
启动子: | SV40 |
复制子: | ori |
质粒大小: | 5874bp |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | Stbl3 |
培养条件: | 37度 |
| 5'测序引物: | 根据序列设计引物 |
3'测序引物: | 根据序列设计引物 |
■ 质粒属性
质粒宿主: | 哺乳细胞,逆病毒 |
质粒用途: | 蛋白表达 |
片段类型: |
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片段物种: |
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原核抗性: | Amp |
真核抗性: | G418
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荧光标记: |
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■ 质粒简介
pLXSN contains elements derived from Moloney murine leukemia virus (MoMuLV) and Moloney murine sarcoma virus (MoMuSV), and is designed for retroviral gene delivery and expression (1–3). Upon transfection into a packaging cell line, pLXSN can transiently express, or integrate and stably express, a transcript containing Y+ (the extended viral packaging signal) the gene of interest, and a selectable marker. The 5' viral LTR in this vector contains promoter/enhancer sequences that control expression of the gene of interest in the multiple cloning site. The SV40 early promoter (PSV40e) controls expression of the neomycin resistance gene (Neor), which allows antibiotic selection in eukaryotic cells. pLXSN also includes the Col E1 origin of replication and E. coli Ampr gene for propagation and antibiotic selection in bacteria.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK747pLXSN逆转录表达质粒.txt
