■ 基本信息
启动子: | CMV |
复制子: | pUC |
终止子: | SV40 poly(A) signal |
质粒分类: | 病毒系列，慢病毒克隆质粒 |
质粒大小: | 8102bp |
原核抗性: | Amp |
筛选标记: | Puro |
克隆菌株: | Stbl3 |
培养条件: | 37℃ |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物:
| CMV-F：CGCAAATGGGCGGTAGGCGTG
|
3'测序引物: | 根据序列设计引物 |
■ 质粒属性
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: |
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片段物种: |
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原核抗性: | Amp |
真核抗性: | 嘌呤霉素
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荧光标记: |
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■ 质粒简介
pLVX-Puro is an HIV-1-based, lentiviral expression vector. Lentiviral particles derived from the vector allow you to express your gene of interest in virtually any cell type, even primary cells. Expression of your gene is driven by the constitutively active human cytomegalovirus immediate early promoter (PCMV IE), located just upstream of the multiple cloning site (MCS), allowing constitutive, high level expression of your protein of interest.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK756pLVX-Puro慢病毒表达质粒.txt
