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产品名称:pLVX-IRES-Neo

货号 规格 价格 订购数量 是否现货
ZK759 1μg(20μl,50ng/μl) 870 - + 有货

基本信息

启动子:

CMV

复制子:

pUC

终止子:

SV40 poly(A) signal

质粒分类:

病毒系列,慢病毒克隆载体

质粒大小:

8269bp

原核抗性:

Amp

真核抗性:

G418

克隆菌株:

Stbl3

培养条件:

37度

表达宿主:

哺乳细胞

诱导方式:

无须诱导,瞬时表达

5'测序引物:
CMV-F:CGCAAATGGGCGGTAGGCGTG

3'测序引物:

根据序列设计引物


质粒属性

质粒宿主:

哺乳细胞,慢病毒

质粒用途:

蛋白表达

片段类型:

ORF

片段物种:

空载体

原核抗性:

Amp

真核抗性:

G418

荧光标记:



质粒简介

pLVX-IRES-Neo is an HIV-1-based, lentiviral expression vector that allows the simultaneous expression of your protein of interest and G418 resistance (Neor) in virtually any mammalian cell type, including primary cells. The vector expresses your protein of interest and Neor from a bicistronic mRNA transcript, allowing G418 resistance to be used as an indicator of transduction efficiency and a marker for selection.

pLVX-IRES-Neo contains all of the viral processing elements necessary for the production of replication-incompetent lentivirus, as well as elements to improve viral titer, transgene expression, and overall vector function. The woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) promotes RNA processing events and enhances nuclear export of viral RNA , leading to increased viral titers from packaging cells. In addition, the vector includes a Rev-response element (RRE), which further increases viral titers by enhancing the transport of unspliced viral RNA out of the nucleus . Finally, pLVX-IRES-Neo also contains a central polypurine tract/central termination sequence element (cPPT/CTS). During target cell infection, this element creates a central DNA flap that increases nuclear import of the viral genome, resulting in improved vector integration and more efficient transduction . The vector also contains a pUC origin of replication and an E. coli ampicillin resistance gene (Ampr) for propagation and selection in bacteria


质粒图谱


质粒序列

质粒序列请下载:ZK759pLVX-IRES-Neo慢病毒表达质粒.txt

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总价格:¥2000