■ 基本信息
启动子: | TRE3G |
复制子: | pUC |
终止子: | SV40 poly(A) signal |
质粒分类: | 病毒系列，慢病毒克隆载体 |
质粒大小: | 7819bp |
原核抗性: | Amp |
真核抗性: | Puro |
克隆菌株: | Stbl3 |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
诱导方式: | 四环素诱导 |
5'测序引物:
| M13R:CAGGAAACAGCTATGACC
|
3'测序引物: | 根据序列设计引物 |
■ 质粒属性
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF
|
片段物种: | 空载体
|
原核抗性: | Amp |
真核抗性: | Puro
|
荧光标记: |
|
■ 质粒简介
When used with any Lenti-X Tet-On 3G or Tet-Express inducible expression system, the pLVX-TRE3G Vector Set allows for lentiviral delivery and inducible expression of your gene of interest in a wide variety of mammalian cells. Target cells that contain a gene of interest under the control of a PTRE3GV promoter will express high levels of your gene of interest, when cultured in the presence of doxycycline (when used with the pLVX-Tet3G Vector and a Tet-On 3G system) or in the presence of the Tet-Express tetracycline transactivator (when used with Tet-Express Systems).
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK773pLVX-TRE3G慢病毒表达质粒.txt
