■ 基本信息
别名: | CD513B-1 |
启动子: | CMV |
复制子: | pUC |
终止子: | SV40 poly(A) signal |
质粒分类: | 病毒系列,慢病毒克隆载体 |
质粒大小: | 8189bp |
原核抗性: | Amp |
真核抗性: | Puro |
克隆菌株: | Stbl3 |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导,瞬时表达 |
5'测序引物: | CMV-F:CGCAAATGGGCGGTAGGCGTG |
3'测序引物: | 根据序列设计引物 |
■ 质粒属性
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF
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片段物种: |
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原核抗性: | Amp |
真核抗性: | Puro
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荧光标记: |
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■ 质粒简介
PCDH-CMV-MCS-EF1-CopGFP-T2A-Puro polyclonal site (MCS) - in MCS is located in the CMV promoter downstream of interest gene cloning.To improve stability and CMV driven transcription translation. SV40 multi - poly adenosine acidification signal to enable the efficiency of transcription and transcription termination. The hybrid / RSV 5ltr promoter provides a high level of viral transcript expression in 293 cells of this full-length producer.
■ 质粒图谱
■ 质粒序列
质粒序列请下载:ZK778pCDH-CMV-MCS-EF1-CopGFP-T2A-Puro慢病毒表达质粒.txt