■ 基本信息
别名: | TRC2-pLKO-Puro;pLKO.5-TRC2-Puro |
启动子: | U6 |
复制子: | pUC |
质粒分类: | 病毒系列，慢病毒干扰载体 |
质粒大小: | 7484bp |
原核抗性: | Amp |
真核抗性: | Puro |
克隆菌株: | Stbl3 |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物:
| U6:ATGGACTATCATATGCTTACCGTA |
3'测序引物: | 根据序列设计引物 |
■ 质粒属性
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 基因沉默 |
片段类型: | shRNA
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片段物种: |
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原核抗性: | Amp |
真核抗性: | Puro
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荧光标记: |
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■ 质粒简介
The MISSION TRC2 Control Vector pLKO-puro is a lentivirus plasmid vector. This vector is in the TRC2 pLKO-puro plasmid backbone, which contains the WPRE. The vector does not contain an shRNA insert and is useful as a negative control in experiments using the TRC2 MISSION shRNA library clones. This allows one to examine the effect of transfection on gene expression and interpret the knockdown effect seen with shRNA clones.
