■ 基本信息
启动子: | U6 |
复制子: | pUC |
终止子: | SV40 poly(A) signal |
质粒分类: | 病毒系列，慢病毒克隆载体 |
质粒大小: | 7757bp |
原核抗性: | Amp |
真核抗性: | Puro |
克隆菌株: | Stbl3 |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物:
| U6:ATGGACTATCATATGCTTACCGTA |
3'测序引物: | 根据序列设计引物 |
■ 质粒属性
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 基因沉默 |
片段类型: | shRNA
|
片段物种: | 空载体
|
原核抗性: | Amp |
真核抗性: | Puro
|
荧光标记: |
|
■ 质粒简介
pLVX-shRNA1 is an HIV-1-based, lentiviral expression vector designed to express a small hair-pin RNA (shRNA) for RNA interference (RNAi) studies. Expression of your shRNA is drivenby the RNA Pol III-dependent, human U6 promoter (PU6), located just upstream of the MCS. pLVX-shRNA1 can either be used as a plasmid expression vector and transfected into cells, or it can be packaged into viral particles and transduced into cells. Lentiviral particles derived from the vector allow the expression of shRNAs in virtually any cell type, including primary cells.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK816pLVX-shRNA1慢病毒干扰质粒.txt
