■ 基本信息
启动子: | Ubc |
复制子: | pUC |
终止子: | SV40 poly(A) signal，bGH poly(A) signal |
质粒分类: | 病毒系列，慢病毒干扰载体 |
质粒大小: | 13320bp |
原核抗性: | Amp |
真核抗性: | Puro |
克隆菌株: | Stbl3 |
培养条件: | 37度 |
表达宿主: | 哺乳细胞 |
5'测序引物: | T7:TAATACGACTCACTATAGGG |
3'测序引物: | 根据序列设计引物 |
■ 质粒属性
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 基因沉默 |
片段类型: | shRNA |
片段物种: |
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原核抗性: | Amp |
真核抗性: | Puro
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荧光标记: |
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■ 质粒简介
The Thermo Scientific Open Biosystems TRIPZ Lentiviral Inducible shRNAmir Library was developed in collaboration with Dr. Greg Hannon (CSHL) and Dr. Steve Elledge (Harvard). This library combines the design advantages of microRNA-adapted shRNA (shRNAmir) with the pTRIPZ lentiviral inducible vector to create a powerful RNAi trigger capable of producing RNAi in most cell types. The vector is engineered to be Tet-On and produces tightly regulated induction of shRNAmir expression in the presence of doxycycline.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK821pTRIPZ慢病毒干扰质粒.txt
